Drabczyk-Pluta, Malgorzata

Models available:

  • Friend virus (FV) infection (mouse) provide an excellent model for the characterisation of immune response against acute and chronic retroviral infection.
  • Immune response against influenza virus (mouse).
  • Immune response against Friend virus induced tumor (mouse).

Cells available:

  • Characterisation of the influence of regulatory T cells (Tregs), inhibitory ligands, and myeloid derived supressor cells (MDSCs) on the regulation of virus-specific T cell immune responses.
  • Immunomodulation and experimenthal immunotherapy directed on above mentioned inhibitory factors in order to restore the virus-specific or tumor-specific T cells.

Technology available:

  • Flow cytometry (up to 13-colours).
  • Isolation of different populations of immune cells and in vitro functional characterisation.
  • In vivo and in vitro analysis of immunomodulatory factors directed at antigen-specific T cells.
  • In vivo analysis of antigen-specific cellular cytotoxicity of T cells.

Key publications related to Mye-EUNITER:

  1. Zelinskyy G, Werner T, Dittmer U. Natural regulatory T cells inhibit production of cytotoxic molecules in CD8⁺ T cells during low-level Friend retrovirus infection. Retrovirology. 2013 Oct 24;10:109. doi: 10.1186/1742-4690-10-109.

  2. Dietze KK, Zelinskyy G, Liu J, Kretzmer F, Schimmer S, Dittmer U. Combining regulatory T cell depletion and inhibitory receptor blockade improves reactivation of exhausted virus-specific CD8+ T cells and efficiently reduces chronic retroviral loads. PLoS Pathog. 2013;9(12):e1003798. doi: 10.1371/journal.ppat.1003798.

  3. Zelinskyy G, Myers L, Dietze KK, Gibbert K, Roggendorf M, Liu J, Lu M, Kraft AR, Teichgräber V, Hasenkrug KJ, Dittmer U. Virus-specific CD8+ T cells upregulate programmed death-1 expression during acute Friend retrovirus infection but are highly cytotoxic and control virus replication. J Immunol. 2011 Oct. 1;187(7):3730-7.

  4. Dietze KK, Zelinskyy G, Gibbert K, Schimmer S, Francois S, Myers L, Sparwasser T, Hasenkrug KJ, Dittmer U. Transient depletion of regulatory T cells in transgenic mice reactivates virus-specific CD8+ T cells and reduces chronic retroviral set points. Proc Natl Acad Sci U S A. 2011 Feb. 8;108(6):2420-5.

  5. Zelinskyy G, Dietze KK, Husecken YP, Schimmer S, Nair S, Werner T, Gibbert K, Kershaw O, Gruber AD, Sparwasser T, Dittmer U. The regulatory T cell response during acute retroviral infection is locally defined and controls the magnitude and duration of the virus-specific cytotoxic T cell response. Blood. 8 Oct. 2009, Vol. 114, No. 15, pp. 3199-3207.