Gregori, Silvia

Models available:

  • Murine human xenograft models, humanized leukemia models
  • Preclinical mirune models of T1D
  • Human samples (blood, tissue)

Cells available:

  • Tolerogenic DC
  • Regulatory T cells

Technology available:

  • Isolation of primary human DC and Tregs from blood using ficoll, magnetic beads and/or flow cytometry sorting
  • Multicolor flow cytometry for phenotyping
  • Cytokine and chemokine secretion assays (flow and Elisa, ELISPOT, Multiplex)
  • Primary T cell cultures, proliferation by CSFE dilution and suppression assays

Key publications related to Mye-EUNITER:

  1. Amodio G, Gregori S. Dendritic cells a double-edge sword in autoimmune responses. Front Immunol. 2012. 3:233. Epub 2012 Aug 2.

  2. Andolfi G, Fousteri G, Rossetti M, Magnani CF, Jofra T, Locafaro G, Bondanza A, Gregori S*, Roncarolo MG*. Enforced IL-10 expression confers type 1 regulatory T cell (Tr1) phenotype and function to human CD4(+) T cells. Mol Ther. 2012. 20(9):1778-90. (*Equal contributors)

  3. Gregori S*, Goudy KS*, Roncarolo MG. The Molecular Mechanisms of Immunosuppression by Type 1 Regulatory T cells. Front Immunol. 2012. 3:30. Epub 2012 Feb 29. (*Equal contributors)

  4. Carosella ED, Gregori S, LeMaoult J. The tolerogenic interplay(s) between HLA-G, APC, and regulatory cells. Blood. 2011. 118(25):6499-505. Epub 2011 Sep 29.

  5. Roncarolo MG, Gregori S, Lucarelli B, Ciceri F, Bacchetta R. Clinical Tolerance in Allogeneic Hematopoietic Stem Cell Transplantation. Immunol Rev. 2011. 241(1):145-63.