Lutz, Manfred

Research interests focus on immune tolerance mediated through myeloid-derived suppressor cells (MDSCs), dendritic cells (DCs), and regulatory T cells (Tregs).

We investigate the molecular signaling requirements of murine monocytes to develop into MDSC and test the potential of in vitro generated MDSCs for therapy of autoimmune models. In addition we work on immune evasion strategies of mycobacteria targeting MDSCs.

We study DCs and Tregs under steady state conditions in mice to learn how tolerance to self-antigens is maintained. The RelB expression in steady state migratory DCs, which transport self antigens from the skin to lymph nodes are in the focus of our current research. Such tolerogenic migratory DCs can be targeted by transcutaneous allergen appication as a variation of hyposensitization in horses.

Further projects include the molecular requirements of immature DCs to convert anergic into regulatory T cells and the immune evasion of Vibrio cholerae by inducing tolerogenic mediators in DCs.

Cells available:

  • Myeloid-derived suppressor cells (MDSC)
  • Tolerogenic dendritic cells

Technology available:

  • Flow cytometry and cell sorting
  • Confocal and two-photon microscopy

Key publications related to Mye-EUNITER:

  1. du Plessis N, Loebenberg L, Kriel M, von Groote-Bidlingmaier F, Ribechini E, Loxton AG, van Helden PD, Lutz MB, Walzl G. Increased frequency of myeloid-derived suppressor cells during active tuberculosis and after recent mycobacterium tuberculosis infection suppresses T-cell function. Am J Respir Crit Care Med. 2013 Sep 15;188(6):724-32.

  2. Ribechini E, Leenen PJ, Lutz MB. Gr-1 antibody induces STAT signaling, macrophage marker expression and abrogation of myeloid-derived suppressor cell activity in BM cells. Eur J Immunol. 2009 Dec;39(12):3538-51.

  3. Ziegler-Heitbrock L, Ancuta P, Crowe S, Dalod M, Grau V, Hart DN, Leenen PJ, Liu YJ, MacPherson G, Randolph GJ, Scherberich J, Schmitz J, Shortman K, Sozzani S, Strobl H, Zembala M, Austyn JM, Lutz MB. Nomenclature of monocytes and dendritic cells in blood. Blood. 2010 Oct 21;116(16):e74-80.

  4. Greifenberg V, Ribechini E, Rössner S, Lutz MB. Myeloid-derived suppressor cell activation by combined LPS and IFN-gamma treatment impairs DC development. Eur J Immunol. 2009 Oct;39(10):2865-76.