Maffia, Pasquale

My major interest in the immune response in cardiovascular diseases. Current research activities address the study of cellular and molecular mechanisms involved in the pathophysiology of atherosclerosis, neointimal formation and stroke. In particular, I am interested in assessing the phenotype and mechanism of action of myeloid regulatory cells homing the diseased vascular wall in atherosclerosis in order to understand how they could be targeted for therapeutic utility.

Models and cell types studied:

Mouse models of atherosclerosis
Rodent models of neointimal formation
Porcine model of in-stent restenosis
Murine models of acute and chronic inflammation

Aortic antigen presenting cells
Plasmacytoid dendritic cells
CD4+ T cells

Technology used:

Confocal and Two photon microscopy
Flow cytometry
Cell culture
Surface-Enhanced Raman Spectroscopy (SERS)

Selected publications relevant to Mye-EUNITER

  1. Maffia P, Zinselmeyer BH, Ialenti A, Kennedy S, Baker AH, McInnes IB, Brewer JM, Garside P. Multiphoton microscopy for 3D imaging of lymphocyte recruitment into Apolipoprotein-E-deficient mouse carotid artery.
    Circulation 2007; 115: e326-8. 
  2. Ialenti A, Grassia G, Gordon P, Maddaluno M, Di Lauro MV, Baker AH, Guglielmotti A, Biondi G, Kennedy S, Maffia P. Inhibition of in-stent restenosis by oral administration of bindarit in porcine coronary arteries.
    Arteriosclerosis Thrombosis and Vascular Biology 2011; 31:2448-2454. 
  3. MacRitchie N, Grassia G, Sabir SR, Maddaluno M, Welsh P, Sattar N, Ialenti A, Kurowska-Stolarska M, McInnes IB, Brewer JM, Garside P, Maffia P. Plasmacytoid Dendritic Cells Play a Key Role in Promoting Atherosclerosis in Apolipoprotein-E-Deficient Mice.
    Arteriosclerosis Thrombosis and Vascular Biology 2012; 32:2569-79. 
  4. Sage A, Murphy DM, Maffia P, Masters LM, Sabir SR, Baker LL, Grassia G, Harrison JE, Ludewig B, Reith W, Hansson GK, Reizis B, Hugues S, Mallat Z. MHC class II-restricted antigen presentation by plasmacytoid dendritic cells drives pro-atherogenic immunity.
    Circulation 2014; 130:1363-73. 
  5. Hu D, Mohanta SK, Yin C, Peng L, Ma Z, Srikakulapu P, Grassia G, MacRitchie N, Dever G, Gordon P, Burton FL, Ialenti A, Sabir SR, McInnes IB, Brewer JM, Garside P, Weber C, Lehmann T, Teupser D, Habenicht L, Beer M, Grabner R, Maffia P, Weih F, Habenicht AJR. ATLOs organize aorta immunity and protect against atherosclerosis via VSMC-LTβRs.
    Immunity 2015; in press