Mye-EUNITER
 

Pekarova, Michaela

Models available:

  • Model of lung inflammation
  • Model of peritonitis
  • Tail bleeding model
  • Heart failure models
  • Human samples (blood – monocytes, neutrophils)
  • Samples from mice (blood, tissues – monocytes/macrophages, neutrophils, bone marrow derived macrophages)
  • Drug administration – isofluran anesthesia and jugular venous cannulation and catheter placement
  • Newly established transgenic animal core facility (MPO-deficient mice) 

Cells available:

  • Monocytes/Macrophages, Neutrophils

Technology available:

  • Spectrophotometers (Nano-drop, Spectra-Rainbow; Specol),
  • Luminometers Orion II (BERTHOLD Detection Systems,
  • Equipment for electrophoresis of nucleic acids, protein electrophoresis and blotting (Bio-Rad),
  • Flow-cytometer FACS CALIBUR system, Becton Dickinson,
  • Multi-detection reader Infinite M200 (TECAN),
  • Fluorescent microscope Olympus IX-70 with camera and software for image analysis,
  • Leica TCS SP5X inverted confocal microscope system (equipped with white laser and acousto-optical beam splitter),
  • High-speed sorter BD Aria II Sorp (equipped with 355, 405, 488&633 nm lasers),
  • Equipment for PCR methodology (gradient thermal cyclers PTC-200, RT cycler 7300, Applied Biosystems, Roche),
  • Perfusion systems for simultaneous culture and observation of cells under flow conditions (Ibidi pump-Ibidi, KimaTM Pump-Cellix),
  • High-resolution ultrasound imaging system (Vevo 2100, VisualSonics Inc),
  • Real-time cell microelectronic sensor-based platform xCELLigence (Roche),
  • Dual-channel aggregometer (Chrono-log)

Key publications related to Mye-EUNITER:

  1. Pekarova M, Kubala L, Martiskova H, Papezikova I, Kralova S, Baldus S, Klinke A, Kuchta R, Kadlec J, Kuchtova Z, Kolarova H, Lojek A. The unique role of dietary L-arginine in the acceleration of peritoneal macrophage sensitivity to bacterial endotoxin. Immunol Res. 2013 May;56(1):73-84. IF= 3,525

  2. Rudolph TK, Rudolph V, Edreira MM, Cole MP, Bonacci G, Schopfer FJ, Woodcock SR, Franek A,Pekarova M, Khoo NK, Hasty AH, Baldus S, Freeman BA. Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient mice. Arterioscler Thromb Vasc Biol. 2010 May;30(5):938-45.

  3. Klinke A, Möller A, Pekarova M, Ravekes T, Friedrichs K, Berlin M, Scheu KM, Kubala L, Kolarova H, Ambrozova G, Schermuly RT, Woodcock SR, Freeman BA, Rosenkranz S, Baldus S, Rudolph V, Rudolph TK. Protective Effects of 10-Nitro-Oleic Acid in a Hypoxia-Induced Murine Model of Pulmonary Hypertension. Am J Respir Cell Mol Biol. 2014 Feb 12. IF= 4,148

  4. von Leitner EC, Klinke A, Atzler D, Slocum JL, Lund N, Kielstein JT, Maas R, Schmidt-Haupt R,Pekarova M, Hellwinkel O, Tsikas D, D'Alecy LG, Lau D, Willems S, Kubala L, Ehmke H, Meinertz T, Blankenberg S, Schwedhelm E, Gadegbeku CA, Böger RH, Baldus S, Sydow K. Pathogenic cycle between the endogenous nitric oxide synthase inhibitor asymmetrical dimethylarginine and the leukocyte-derived hemoprotein myeloperoxidase. Circulation. 2011 Dec 13;124(24):2735-45.

  5. Viačková D, Pekarová M, Crhák T, Búcsaiová M, Matiašovic J, Lojek A, Kubala L. Redox-sensitive regulation of macrophage-inducible nitric oxide synthase expression in vitro does not correlate with the failure of apocynin to prevent lung inflammation induced by endotoxin. Immunobiology. 2011 Apr;216(4):457-65.

Contact details:

Institute of Biophysics
Academy of Sciences of the Czech Republic, v.v.i. Kralovopolska 135
CZ-612 65 Brno
Czech Republic
Phone: +420 - 541 517 207
Fax: +420 - 541 211 293
pekarovam@ibp.cz