Scapini, Patrizia

Main research interest is the investigation of the contribution of neutrophils, and/or neutrophil-specific signalling pathways, in inflammatory diseases including autoimmune diseases and cancer. In relationship with the Mye-EUNITER program, several effort are dedicated to the understanding of the role of neutrophils in patients suffering from several types of cancer, including lymphomas [such as non-Hodgkin lymphoma (NHL), HL, multiple myeloma, etc] or solid tumors (such as colon carcinoma, lung carcinoma, etc) or in mouse models of cancer.

Models available:

Patients suffering from inflammatory/autoimmune diseases (including psoriasis, rheumatoid arthritis and systemic lupus erythematosus, SLE) and cancer. Mouse models of cancer and inflammatory/autoimmune diseases (psoriasis and SLE) performed in several conditional knockout mouse strains carrying the specific deletion in neutrophils of crucial molecules involved in the modulation of the functions of these cells.

Cells available:

Neutrophils, monocytes/macrophages

Technology available:

Techniques for gene expression analysis; flow cytometry and cell sorting; immunocytochemistry/mmunofluorescence; ELISA and Bio-Plex assays; Western-blot; functional assays (e.g. evaluation of cell apoptosis, degranulation, production of reactive oxygen species, cell cycle and proliferation, cytokine production)

Key publications related to Mye-EUNITER:

  1. Scapini P, Cassatella MA. Social networking of human neutrophils within the immune system. Blood. 2014 124(5):710-9
  2. Lamagna C, Scapini P, van Ziffle JA, DeFranco AL, Lowell CA. Hyperactivated MyD88 signaling in dendritic cells, through specific deletion of Lyn kinase, causes severe autoimmunity and inflammation. Proc Natl Acad Sci U S A. 2013 Aug 27;110(35):E3311-20.
  3. Scapini P., Lamagna P, Hu Y, Lee K, Tang Q, DeFranco AL, & Lowell CA. 2011. B cell-derived IL-10 suppresses inflammation and autoimmunity in Lyn-deficient mice. PNAS. 108:E823-832;
  4. Scapini P., Hu Y, Chu C.L., Migone T.S., Defranco A.L., Cassatella M.A., Lowell C.A. 2010. Myeloid cells, BAFF, and IFN-{gamma} establish an inflammatory loop that exacerbates autoimmunity in Lyn-deficient mice. J. Exp. Med. 207: 1757-1773;
  5. Scapini P., Pereira S., Zhang H., Lowell CA. 2009 Multiple roles of Lyn kinase in myeloid cell signaling and function. Immunol. Rev. 228:1-17;